Easy English Platinum Activation Code [portable Edition]
Easy English Platinum Activation Code [Portable edition]
English Platinum Activation Code [Portable Edition]
View the License Key of Easy English Platinum Activation Code [Portable edition]
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A cellobiohydrolase gene (cbh1) was found in Aspergillus usamii JCM 4731 by PCR-amplification of the cbh1 gene using the degenerate primers deduced from sequencing of endoglucanase genes of A. japonicus. Gene-disruption experiments using a β-lactamase plasmid showed that the cellobiohydrolase activity was dependent on the presence of a β-lactamase gene that had inserted into the cbh1 structural gene. A cellobiohydrolase of 507 amino acids was synthesized in Escherichia coli and this protein was used as an antigen in antibody production. Purified antibodies were then used to show that CBH1 is a secretory protein which is expressed and secreted into the culture medium. CBH1
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Amperometry is a powerful detection technique. Amperometry has been used to sense the presence of biologically active molecules, such as glucose, pH, lactate, iron, and oxygen. Amperometry also has been used to measure the concentration of drugs, such as metoprolol, clarithromycin, warfarin, and isoniazid. Amperometric detection can be used in single-use tests.
The measurement of ions directly in a biological sample can also be of interest. For example, the measurement of potassium ion concentration in a biological sample can be a useful measurement to identify a diabetic patient who is experiencing either hypo- or hyper-kalemia.
Amperometric detection is based on the fact that the electrogenic oxidation of analytes by electrodes produces a current. The current is measured through a circuit, and an analyte concentration can be determined by measuring the magnitude of the measured current. The analyte, however, must be oxidized to produce a measurable current.
One method of sensing an analyte involves using enzymes to oxidize the analyte. For example, glucose is oxidized by glucose oxidase, which produces an electron and an oxygen molecule. The oxygen molecule can be combined with oxygen to form water, or reduced to form water-soluble hydroxyl free radicals.
Alternatively, the
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